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KMID : 0360319950270020195
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Journal of Korean Cancer Research Association
1995 Volume.27 No. 2 p.195 ~ p.201
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Amplification of the c-erbB-2 Proto-Oncogene in Primary Human Gastric Cancers
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최철원
서재홍/김병수/신상원/김준석/목영재/김종석/구범환
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Abstract
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The c-erbB-2 proto-oncogene has been found to be amplified in many human tumors including breast, stomach, kidney and lung cancers. In order to evaluate the amplification status of c-erbB-2 proto-oncogene in stomach cacer which is the most common
cancer
in Korea, fortyfive sets of specimens including tumor tissue and normal stomach tissue(5 cm at least away from primary tumor, histologically normal) were measured by Southern hybridization technique. Ten micrograms of EcoRI-digested DNA was
electrophoresed in an 0.8% agarose gel and transferred to a nylon memebrane and hybridized with a c-erbB-2 probe, washed under stringent conditions and autoradiographed against HyperfilmTM with intensifying screens at 70℃for days. In the
results.
Two
of the 45 gastric carcinomas(4.4%) were amplified 2-to-4-fold. Thus, the amplified c-erbB-2 gene may have a role in the evolution of gastric carcinoma, as dose in some carcinoma.
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